A.I. BURNASYAN FMBC CLINICAL BULLETIN

ISSN 2782-6430 (print)

State Research Center −Burnasyan Federal Medical Biophysical
Center of Federal Medical Biological Agency

The journal is published in Russian.
Format – A4.
The periodicity of the journal is 4 times a year.

Issue №2 2025 год

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A.I. Burnasyan  FMBC clinical bulletin. 2025 № 1

Yu.G.Lutsenko ., D.R. Sycheva

Pathomorphological Aspects of the Regenerative Process of Wounds Caused
by the Use of Collagen Wound Coatings in Patients with Diabetic Foot Syndrome

Federal State Budgetary Educational Institution of Higher Education “Donetsk State Medical University named after M. Gorky”, Donetsk, Russia

Contact person: Sycheva Darya Romanovna:idarya.hardy@mail.ru

Abstract
Purpose: to evaluate the morphological features of the course of reparative processes in patients with diabetic foot syndrome (DFS) due to the use of collagen wound coatings.
Material and methods. the paper presents a comparative retrospective analysis of the histological material of wound tissues of 112 DFS patients. Morphological examination of the material was carried out using a microscope “Micros MC 50”, a lens “WF EWF 10x/18”. The studied material is divided into two groups: the main one is 62 micro-preparations of the wound tissue of DFS patients, collagen wound coatings (“Green Oak”, RF) were used in the complex treatment of wound defects; The control group consisted of 60 micro–preparations of wound tissues of DFS patients, local treatment of wound defects consisted of daily dressings with antiseptics and ointments.
Results and discussion. fnalysis of the obtained results showed that the use of collagen wound coatings affects each phase of the reparative process of chronic wounds of DFS patients, contributes to a faster transition from the alternativeexudative stage of inflammatory reaction in proliferative and epithelial phase; collagen absorbs wound exudate, performing a drainage function, contributing to acceleration of wound cleansing processes, reduces inflammatory reaction, normalizes the functioning of microcirculatory vessels, stabilizing blood supply in the area of wound defect and reducing hypoxia, leads to the activation of reparative processes; the use of collagen wound coatings activates the process of leukocyte migration, significantly increases the number of macrophages, promoting increased expression of proinflammatory cytokines, speeds up the process of angiogenesis, migration and proliferation of fibroblasts – granulation tissue in the wounds undergoes a maturation process much faster than the group of patients, treated with daily bandages with antiseptics and ointments.
Conclusion. the use of collagen wound coatings in the complex treatment of patients with purulent necrotic DFS forms can significantly accelerate the course of each phase of the reparative process in wound defects due to the influence of this technique granulation tissue is able to pass the process of maturation in a shorter period of time, allowing to reduce the time of preparation for plastic closure from 20-22 to 12-16 days.

Keywords: diabetes mellitus, diabetic foot, collagen wound coverings, collagen, macrophages, fibroblasts, regeneration

For citation: Lutsenko YG, Sycheva DR. Pathomorphological Aspects of the Regenerative Process of Wounds Caused by the Use of Collagen Wound Coatings in Patients with Diabetic Foot Syndrome. A.I. Burnasyan Federal Medical Biophysical Center Clinical Bulletin. 2025.1:49-56. (In Russian) DOI: 10.33266/2782-6430-2025-1-49-56

 

REFERENCES

  1. Sindrom Diabeticheskoy Stopy = Diabetic Foot Syndrome. Clinical Guidelines. Russian Association of Endocrinologists, Moscow Association of Surgeons. Moscow Publ., 2015. 46 p. (In Russ.).
  2. Galstyan G.R., Tokmakova A.Yu., Yegorova D.N., Mitish V.A., et al. Clinical Recommendations for the Diagnosis and Treatment of Diabetic Foot Syndrome. Rany i Ranevyye Infektsii. Zhurnal imeni professora B.M. Kostyuchenka = Journal Named after Professor B.M. Kostyuchenko. 2015;2:61-83 (In Russ.).
  3. International Diabetes Federation. Diabetes Atlas. Brussels, 2017. 148 p.
  4. Davidenko O.P. Diabetic Foot Syndrome. Its Role and Place in Modern Diabetology (Literature Review). Meditsina i Obrazovaniye v Sibiri: Elektronnyy Zhurnal = Medicine and Education in Siberia: Electronic Journal. 2014;5:16-23 (In Russ.).
  5. Li X., Li D., Wikstrom J. D., et al. Mi- croRNA-132 Promotes Fibroblast Migra- Tion Via Regulating RAS p21 Protein Activator 1 in Skin Wound Healing. Sci Rep. 2017;7;1:7797.
  6. Berchenko G.N., Shekhter A.B., Nikolayev A.V., et al. Features of Healing of Purulent Wounds in Patients Treated with Collagen Preparations (Histochemical and Electron Microscopic Examination). Arkhiv Patologii = Archive of Pathology. 1985;12:37-44 (In Russ.).
  7. Lisitsyn Yu.P. Istoriya Meditsiny = History of Medicine. Moscow, GEOTAR-Media Publ., 2016. 400 p. (In Russ.).
  8. Vaisov A.Sh., Dronov A.F., Istranov L.P., Rudenko T.G. The Use of Collagen Preparations in the Treatment of Skin Wounds. Meditsinskiy Zhurnal Uzbekistana = Medical Journal of Uzbekistan. 1975;8:56-64 (In Russ.).
  9. Malinin V.V., Burakova M.A., Sidorova N.D., et al. Collagen Sponge “Cytothymacol”: A Wound-Healing Stimulator. Pharm Chem J. 1998;32:622–624.
  10. Khil’kin A.M., Shekhter A.B., Lemenev V.L., Dronov A.F. Treatment of Scalped Wounds and Skin Burns with Collagen Films in an Experiment. Eksperimental’naya Khirurgiya = Experimental Surgery. 1972;6:37-41 (In Russ.).
  11. Abdullayev R.N. Complex Treatment of Purulent-Necrotic Lesions of the Lower Extremity in Patients with Diabetic Foot Syndrome. Novyy Den’ v Meditsine = New Day in Medicine. 2021;2;34:413-418 (In Russ.).
  12. Belik B.M., Chumburidze I.P., Shtil’man M.Yu., Yavruyan O.A., Savchenko Yu.P. Complex Treatment of Patients with Purulent-Necrotic Complications in the Neuropathic Form of Diabetic Foot Syndrome. Khirurgiya. Zhurnal imeni N.I. Pirogova = Surgery. Journal Named after N.I. Pirogov. 2020;4:81-87 (In Russ.).
  13. Belova S.V., Babushkina I.V., Gladkova Ye.V., Mamonova I.A. Possibility of Soft Tissue Reparation in Experimental Purulent Wound Conditions. Eksperimental’naya i Klinicheskaya Farmakologiya = Experimental and Clinical Pharmacology. 2016;79;7:35-38 (In Russ.).
  14. Werner S. Regulation of Wound Healing by Growth Factors and Cytokines. Physiol. Rev. 2003;83:835-870.
  15. Velnar Т., Bailey Т., Smrkolj V. The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms. J. Int. Med. Res. 2009;37:1528–1542.
  16. Nair R, Sevukarajan M, Mohammed T, Badivaddin CK, Kumar A. Collagen Based Drug Delivery Systems: a Review. J Innov Trends Pharm Sci. 2010;1:288-304.
  17. Doillon CJ, Silver FH. Collagen Based Wound Dressing Effects of Hyaluronic Acid and Fibronectin on Wound Healing. Biomaterials. 1986;7;1:3-8. doi:10.1016/0142-9612(86)90080-3.

Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors. 
Article received: 21.11.2024. Accepted for publication: 15.12.2024

© 2025 Scientific journal - "Clinical Bulletin of the FMBС im. A.I. Burnasyan"
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